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Sci Rep. 2015 Dec 17;5:18393. doi: 10.1038/srep18393.

Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field.

Author information

1
Department of Maxillofacial Orthognathics, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8510, JAPAN.
2
Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
3
Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, JAPAN.
4
Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
5
Organ Technologies Inc., Tokyo, 108-0074, JAPAN.
6
RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, JAPAN.
7
Department of Clinical Pathophysiology, Tokyo Dental College, Chiyoda-ku, Tokyo, 101-0061, JAPAN.
8
Department of Physics and Mathematics, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, Kanagawa, 252-5258, JAPAN.

Abstract

The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force.

PMID:
26673152
PMCID:
PMC4682098
DOI:
10.1038/srep18393
[Indexed for MEDLINE]
Free PMC Article

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