Format

Send to

Choose Destination
Vet Immunol Immunopathol. 2015 Dec 15;168(3-4):140-6. doi: 10.1016/j.vetimm.2015.10.015. Epub 2015 Nov 3.

Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).

Author information

1
Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, Hannover 30559, Germany; Center for Systems Neuroscience, Bünteweg 2, Hannover 30559, Germany.
2
Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, Bünteweg 9, Hannover 30559, Germany.
3
Center for Systems Neuroscience, Bünteweg 2, Hannover 30559, Germany; Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, Bünteweg 9, Hannover 30559, Germany.
4
Zoo Osnabrück gGmbH, Klaus-Strick-Weg 12, Osnabrück 49082, Germany.
5
Hannover Adventure Zoo, Adenauerallee 3, 30175 Hannover, Germany.
6
Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, Hannover 30559, Germany.
7
Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, Hannover 30559, Germany; Center for Systems Neuroscience, Bünteweg 2, Hannover 30559, Germany. Electronic address: Wolfgang.Baumgaertner@tiho-hannover.de.

Abstract

The raccoon (Procyon lotor) is a highly adaptable carnivore that has rapidly conquered Europe over the last decades and represents a potential candidate as pathogen reservoir, bearing the risk for transmission of infectious agents, as zoonosis or spill-over, to other wild life and domestic animals and man. Comprehensive investigations of infectious diseases in raccoons require a detailed knowledge of the participating immune cell populations. To close this gap of knowledge, various antibodies were tested for cross-reactivity with leukocytes in lymphoid organs and peripheral blood of raccoons using immunohistochemistry and flow cytometry, respectively. Eight out of 16 antibodies, directed against CD3, CD79α, Pax-5, IgG, CD44, MHC class II, myeloid/histiocyte antigen (MAC387), and Iba-1 exhibited a specific immunoreaction with cells in distinct anatomical compartments in formalin-fixed paraffin-embedded lymphoid tissues. Flow cytometric analysis revealed that 7 out of 18 antibodies directed against CD11c, CD14, CD21, CD44, CD79α, MHC class I and II cross-reacted with peripheral blood-derived raccoon leukocytes. Summarized, the usefulness of several cross-reacting antibodies was determined for the characterization of raccoon immune cells in immunohistochemistry and flow cytometry, offering the opportunity to study the raccoon immune system under normal and diseased conditions.

KEYWORDS:

Cross-reacting antibodies; Density gradient centrifugation; Flow cytometry; Immunohistochemistry; Leukocytes; Raccoon

PMID:
26672912
DOI:
10.1016/j.vetimm.2015.10.015
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center