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Plant Physiol. 2016 Mar;170(3):1284-99. doi: 10.1104/pp.15.01308. Epub 2015 Dec 15.

Glutaredoxin S15 Is Involved in Fe-S Cluster Transfer in Mitochondria Influencing Lipoic Acid-Dependent Enzymes, Plant Growth, and Arsenic Tolerance in Arabidopsis.

Author information

1
ARC Centre of Excellence in Plant Energy Biology, M316, Faculty of Science, The University of Western Australia, Crawley, 6009 Western Australia, Australia (E.S., J.G., C.C., R.F., A.H.M.); and ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, LaTrobe University, Bundoora, 3086 Victoria, Australia (J.W.).
2
ARC Centre of Excellence in Plant Energy Biology, M316, Faculty of Science, The University of Western Australia, Crawley, 6009 Western Australia, Australia (E.S., J.G., C.C., R.F., A.H.M.); and ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, LaTrobe University, Bundoora, 3086 Victoria, Australia (J.W.) harvey.millar@uwa.edu.au.

Abstract

Glutaredoxins (Grxs) are small proteins that function as oxidoreductases with roles in deglutathionylation of proteins, reduction of antioxidants, and assembly of iron-sulfur (Fe-S) cluster-containing enzymes. Which of the 33 Grxs in Arabidopsis (Arabidopsis thaliana) perform roles in Fe-S assembly in mitochondria is unknown. We have examined in detail the function of the monothiol GrxS15 in plants. Our results show its exclusive mitochondrial localization, and we are concluding it is the major or only Grx in this subcellular location. Recombinant GrxS15 has a very low deglutathionylation and dehydroascorbate reductase activity, but it binds a Fe-S cluster. Partially removing GrxS15 from mitochondria slowed whole plant growth and respiration. Native GrxS15 is shown to be especially important for lipoic acid-dependent enzymes in mitochondria, highlighting a putative role in the transfer of Fe-S clusters in this process. The enhanced effect of the toxin arsenic on the growth of GrxS15 knockdown plants compared to wild type highlights the role of mitochondrial glutaredoxin Fe-S-binding in whole plant growth and toxin tolerance.

PMID:
26672074
PMCID:
PMC4775112
DOI:
10.1104/pp.15.01308
[Indexed for MEDLINE]
Free PMC Article

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