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Microscopy (Oxf). 2016 Feb;65(1):43-56. doi: 10.1093/jmicro/dfv369. Epub 2015 Dec 15.

Automated data collection in single particle electron microscopy.

Author information

1
The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
2
The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA.
3
The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA bcarr@nysbc.org.

Abstract

Automated data collection is an integral part of modern workflows in single particle electron microscopy (EM) research. This review surveys the software packages available for automated single particle EM data collection. The degree of automation at each stage of data collection is evaluated, and the capabilities of the software packages are described. Finally, future trends in automation are discussed.

KEYWORDS:

automation; data collection; high throughput; single particle electron microscopy; software packages; target acquisition

PMID:
26671944
PMCID:
PMC4749047
DOI:
10.1093/jmicro/dfv369
[Indexed for MEDLINE]
Free PMC Article

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