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Cell Rep. 2015 Dec 15;13(10):2098-106. doi: 10.1016/j.celrep.2015.11.011. Epub 2015 Dec 6.

In Vivo Monosynaptic Excitatory Transmission between Layer 2 Cortical Pyramidal Neurons.

Author information

1
Department of Neuroscience, Max Delbrück Center for Molecular Medicine (MDC), Berlin-Buch, Robert-Rössle-Strasse 10, 13092 Berlin, Germany; Cluster of Excellence NeuroCure, Neuroscience Research Center, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
2
Department of Neuroscience, Max Delbrück Center for Molecular Medicine (MDC), Berlin-Buch, Robert-Rössle-Strasse 10, 13092 Berlin, Germany; Cluster of Excellence NeuroCure, Neuroscience Research Center, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany; Department of Biology, Institute for Theoretical Biology, Humboldt-Universität zu Berlin, Philippstrasse 13, 10115 Berlin, Germany.
3
Department of Neuroscience, Max Delbrück Center for Molecular Medicine (MDC), Berlin-Buch, Robert-Rössle-Strasse 10, 13092 Berlin, Germany; Cluster of Excellence NeuroCure, Neuroscience Research Center, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: james.poulet@mdc-berlin.de.

Abstract

Little is known about the properties of monosynaptic connections between identified neurons in vivo. We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo to investigate excitatory monosynaptic transmission in the hyperpolarized downstate. We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a small minority >1 mV. The coefficient of variation (CV = 0.74) could largely be explained by the presence of synaptic failures (22%). Both the CV and failure rates were reduced with increasing amplitude. The mean paired-pulse ratio was 1.15 and positively correlated with the CV. Our approach will help bridge the gap between connectivity and function and allow investigations into the impact of brain state on monosynaptic transmission and integration.

PMID:
26670044
PMCID:
PMC4688033
DOI:
10.1016/j.celrep.2015.11.011
[Indexed for MEDLINE]
Free PMC Article

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