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Cell Stem Cell. 2016 Jan 7;18(1):144-55. doi: 10.1016/j.stem.2015.10.004. Epub 2015 Dec 5.

Phase I/II Trial of StemRegenin-1 Expanded Umbilical Cord Blood Hematopoietic Stem Cells Supports Testing as a Stand-Alone Graft.

Author information

1
Blood and Marrow Transplant Program, University of Minnesota, Minneapolis, MN 55455, USA; Molecular and Cellular Therapeutics Facility, University of Minnesota, Minneapolis, MN 55108, USA. Electronic address: wagne002@umn.edu.
2
Blood and Marrow Transplant Program, University of Minnesota, Minneapolis, MN 55455, USA.
3
Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA.
4
Molecular and Cellular Therapeutics Facility, University of Minnesota, Minneapolis, MN 55108, USA.
5
Novartis Institutes for BioMedical Research, Basel 4000, Switzerland.

Abstract

Clinical application of umbilical cord blood (UCB) as a source of hematopoietic stem cells for transplantation is limited by low CD34+ cell dose, increased risk of graft failure, and slow hematopoietic recovery. While the cell dose limitation is partially mitigated by using two UCB units, larger-dosed single units would be preferable. We have evaluated the feasibility and safety of StemRegenin-1 (SR-1), an aryl hydrocarbon receptor antagonist that expands CD34+ cells, by placing one of the two units in expansion culture. SR-1 produced a 330-fold increase in CD34+ cells and led to engraftment in 17/17 patients at a median of 15 days for neutrophils and 49 days for platelets, significantly faster than in patients treated with unmanipulated UCB. Taken together, the marked expansion, absence of graft failure, and enhanced hematopoietic recovery support testing of SR-1 expansion as a stand-alone graft and suggest it may ameliorate a limitation of UCB transplant.

PMID:
26669897
PMCID:
PMC4881386
DOI:
10.1016/j.stem.2015.10.004
[Indexed for MEDLINE]
Free PMC Article

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