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BMC Complement Altern Med. 2015 Dec 15;15(1):435. doi: 10.1186/s12906-015-0959-y.

Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays.

Author information

1
National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico. claudia.delgadillop@incmnsz.mx.
2
National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico.
3
Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
4
Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.

Abstract

BACKGROUND:

Obesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases. Oxidative damage is another side effect of obesity. Antioxidant activity from plant components regulates at some extent this imbalance. Main goal of the present study was to determine the antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri (AS) and Acacia farnesiana (AF) pods extracts.

METHODS:

To evaluated antioxidant activity and radical scavenging capacity of AS and AF extracts, two experiments were performed: 1) pods extracts were challenged against H2O2 using kidney cells in an in vitro assay; and 2) (Meriones unguiculatus) was employed in an in vivo assay to observe the effect of pods extracts on scavenging properties in plasma.

RESULTS:

Both pods extracts presented an important protective effect on radical scavenging capacity against ABTS• + and DPPH(+), and also in TBARS formation in vitro. Vegetal pods extracts did not induce any pro-oxidative effect when added to kidney cells in DMEM. Cells damage in DMEM with addition of H2O2 was significantly higher than those when vegetal pods extracts were added at 50 (P < 0.05) or 200 ppm (P < 0.001). Plasma scavenging properties presented an important dose-dependent positive effect in those groups where pods extracts were administered.

CONCLUSIONS:

The antioxidant protection of the acacia pods extracts reported in this study suggests the possible transference of antioxidant components and protective effects to animal products (milk, meat, and by-products) from Acacia pods when this vegetation is included in the diet. In order to evaluate, the possible transference of theirs antioxidant components to animal products, the incorporation of these non-conventional resources to ruminant feeding is a good opportunity of study. Profiling of Acacia farnesiana pods extract is necessary to identify the responsible bioactive compounds of protective properties.

PMID:
26669565
PMCID:
PMC4678613
DOI:
10.1186/s12906-015-0959-y
[Indexed for MEDLINE]
Free PMC Article

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