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Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):15982-7. doi: 10.1073/pnas.1514265112. Epub 2015 Dec 14.

Recombination patterns in maize reveal limits to crossover homeostasis.

Author information

1
Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853;
2
Génétique Quantitative et Evolution-Le Moulon, Institut National de la Recherche Agronomique/Université Paris-Sud/CNRS/AgroParisTech, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France.
3
Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853; wp45@cornell.edu.

Abstract

During meiotic recombination, double-strand breaks (DSBs) are formed in chromosomal DNA and then repaired as either crossovers (COs) or non-crossovers (NCOs). In most taxa, the number of DSBs vastly exceeds the number of COs. COs are required for generating genetic diversity in the progeny, as well as proper chromosome segregation. Their formation is tightly controlled so that there is at least one CO per pair of homologous chromosomes whereas the maximum number of COs per chromosome pair is fairly limited. One of the main mechanisms controlling the number of recombination events per meiosis is CO homeostasis, which maintains a stable CO number even when the DSB number is dramatically altered. The existence of CO homeostasis has been reported in several species, including mouse, yeast, and Caenorhabditis elegans. However, it is not known whether homeostasis exists in the same form in all species. In addition, the studies of homeostasis have been conducted using mutants and/or transgenic lines exhibiting fairly severe meiotic phenotypes, and it is unclear how important homeostasis is under normal physiological conditions. We found that, in maize, CO control is robust only to ensure one CO per chromosome pair. However, once this limit is reached, the CO number is linearly related to the DSB number. We propose that CO control is a multifaceted process whose different aspects have a varying degree of importance in different species.

KEYWORDS:

crossing-over; crossover homeostasis; double-strand breaks; meiosis; recombination

PMID:
26668366
PMCID:
PMC4703008
DOI:
10.1073/pnas.1514265112
[Indexed for MEDLINE]
Free PMC Article

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