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Reproduction. 2016 Mar;151(3):239-51. doi: 10.1530/REP-15-0451. Epub 2015 Dec 14.

SKAP, an outer kinetochore protein, is required for mouse germ cell development.

Author information

1
CNRSIGH (UPR1142), 141 rue de la Cardonille, 34396 Montpellier, FranceCNRSCRBM (UMR5237), 1919 route de Mende, 34293 Montpellier, FranceINRADépartement Biologie et Amélioration des Plantes, route de Saint-Cyr, 78026 Versailles, FranceCollège de FranceCIRB (UMR CNRS 7241/INSERM- U1050), 11 Place Marcelin Berthelot, 75005 Paris, FranceDepartamento de BiologíaFacultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain corinne.grey@igh.cnrs.fr bernard.de-massy@igh.cnrs.fr.
2
CNRSIGH (UPR1142), 141 rue de la Cardonille, 34396 Montpellier, FranceCNRSCRBM (UMR5237), 1919 route de Mende, 34293 Montpellier, FranceINRADépartement Biologie et Amélioration des Plantes, route de Saint-Cyr, 78026 Versailles, FranceCollège de FranceCIRB (UMR CNRS 7241/INSERM- U1050), 11 Place Marcelin Berthelot, 75005 Paris, FranceDepartamento de BiologíaFacultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

Abstract

In sexually reproducing organisms, accurate gametogenesis is crucial for the transmission of genetic material from one generation to the next. This requires the faithful segregation of chromosomes during mitotic and meiotic divisions. One of the main players in this process is the kinetochore, a large multi-protein complex that forms at the interface of centromeres and microtubules. Here, we analyzed the expression profile and function of small kinetochore-associated protein (SKAP) in the mouse. We found that two distinct SKAP isoforms are specifically expressed in the germline: a smaller isoform, which is detected in spermatogonia and spermatocytes and localized in the outer mitotic and meiotic kinetochores from metaphase to telophase, and a larger isoform, which is expressed in the cytoplasm of elongating spermatids. We generated SKAP-deficient mice and found that testis size and sperm production were severely reduced in mutant males. This phenotype was partially caused by defects during spermatogonia proliferation before entry into meiosis. We conclude that mouse SKAP, while being dispensable for somatic cell divisions, has an important role in the successful outcome of male gametogenesis. In germ cells, analogous to what has been suggested in studies using immortalized cells, SKAP most likely stabilizes the interaction between kinetochores and microtubules, where it might be needed as an extra safeguard to ensure the correct segregation of mitotic and meiotic chromosomes.

PMID:
26667018
PMCID:
PMC4738695
DOI:
10.1530/REP-15-0451
[Indexed for MEDLINE]
Free PMC Article

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