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Plant Cell Environ. 2017 Apr;40(4):491-508. doi: 10.1111/pce.12686. Epub 2016 Jan 18.

Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

Author information

1
Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.
2
Fédération de Recherche 3450, Agrobiosciences, Interactions et Biodiversité, CNRS, 31326, Castanet-Tolosan, France.
3
Institut de Pharmacologie et de Biologie Structurale - CNRS, Université de Toulouse, 205 route de Narbonne,, 31077, Toulouse, France.
4
INRA, UMR 1347 Agroécologie, Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.

Abstract

Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both the protein and transcript levels. Interestingly, only a small proportion of the overall NtCdc48 population appeared to be S-nitrosylated. Using gel filtration in native conditions, we confirmed that NtCdc48 was present in its hexameric active form. An immunoprecipitation-based strategy following my mass spectrometry analysis led to the identification of about a hundred NtCdc48 partners and underlined its contribution in cellular processes including targeting of ubiquitylated proteins for proteasome-dependent degradation, subcellular trafficking and redox regulation. Finally, the analysis of cryptogein-induced events in NtCdc48-overexpressing cells highlighted a correlation between NtCdc48 expression and hypersensitive cell death. Altogether, this study identified NtCdc48 as a component of cryptogein signalling and plant immunity.

KEYWORDS:

Cdc48 partners; NtCdc48; cell death; cryptogein; defence responses

PMID:
26662183
DOI:
10.1111/pce.12686
[Indexed for MEDLINE]

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