Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells

Chinnapaka Somaiah; Atul Kumar; Darilang Mawrie; Amit Sharma; Suraj Dasharath Patil; Jina Bhattacharyya; Rajaram Swaminathan; Bithiah Grace Jaganathan

doi:10.1371/journal.pone.0145068

Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells

PLoS One. 2015 Dec 14;10(12):e0145068. doi: 10.1371/journal.pone.0145068. eCollection 2015.

Authors

Chinnapaka Somaiah¹, Atul Kumar¹, Darilang Mawrie¹, Amit Sharma¹, Suraj Dasharath Patil¹, Jina Bhattacharyya², Rajaram Swaminathan³, Bithiah Grace Jaganathan¹

Affiliations

¹ Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
² Department of Hematology, Gauhati Medical College Hospital, Assam, India.
³ Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.

Abstract

Mesenchymal stem cells (MSC) can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Although several studies have been done to understand the conditions that promote proliferation, differentiation and migration of MSC in vitro and in vivo, still there is no clear understanding on the effect of non-cellular bio molecules. Of the many factors that influence the cell behavior, the immediate cell microenvironment plays a major role. In this context, we studied the effect of extracellular matrix (ECM) proteins in controlling cell survival, proliferation, migration and directed MSC differentiation. We found that collagen promoted cell proliferation, cell survival under stress and promoted high cell adhesion to the cell culture surface. Increased osteogenic differentiation accompanied by high active RHOA (Ras homology gene family member A) levels was exhibited by MSC cultured on collagen. In conclusion, our study shows that collagen will be a suitable matrix for large scale production of MSC with high survival rate and to obtain high osteogenic differentiation for therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Adipogenesis / drug effects
Bone Marrow Cells / cytology
Cell Adhesion / drug effects
Cell Differentiation / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Cells, Cultured
Collagen / chemistry
Collagen / pharmacology*
Extracellular Matrix Proteins / metabolism
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Microscopy, Fluorescence
Mitochondria / metabolism
Osteogenesis / drug effects
RNA, Messenger / metabolism
rhoA GTP-Binding Protein / genetics
rhoA GTP-Binding Protein / metabolism

Substances

Actins
Extracellular Matrix Proteins
RNA, Messenger
RHOA protein, human
Collagen
rhoA GTP-Binding Protein

Grants and funding

CS, AK, DM are supported by fellowship from Ministry of Human Resource Development, Govt. of India. This work was supported by funds from Department of Science and Technology SR/FT/LS-97/2009 (DST), Govt. of India and Department of Biotechnology (DBT), Govt. of India under RGYI (Rapid Grant for Young Investigator) scheme BT/PRI/5091/GBD/27/310/2011 to BGJ (http://www.dbtindia.nic.in/). TIRF measurement was performed in facility supported by DBT to RS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.