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FEBS Lett. 1989 Jul 3;250(2):575-9.

A cDNA clone encoding the precursor for a 10.2 kDa photosystem I polypeptide of barley.

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Department of Plant Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.


Two cDNA clones for the barley photosystem I polypeptide which migrates with an apparent molecular mass of 9.5 kDa on SDS-polyacrylamide gels have been isolated using antibodies and an oligonucleotide probe. The determined N-terminal amino acid sequence for the mature polypeptide confirms the identification of the clones. The 644 base-pair sequence of one of the clones contains one large open reading frame coding for a 14,882 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10 193 Da. The hydropathy plot of the polypeptide shows one membranespanning region with a predicted alpha-helix secondary structure. The gene for the 9.5 kDa polypeptide has been designated PsaH.

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