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Methods Mol Biol. 2016;1383:167-75. doi: 10.1007/978-1-4939-3252-8_18.

Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Author information

1
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, 84108, UT, USA.
2
ARUP Laboratories, Salt Lake City, UT, USA.
3
Department of Pathology, University of Utah Health Sciences Center, School of Medicine, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, 84108, UT, USA. kamisha.johnson-davis@aruplab.com.

Abstract

Ethyl glucuronide and ethyl sulfate are minor conjugated metabolites of ethanol that can be detected in urine for several days after last ingestion of ethanol. The monitoring of ethanol use has both clinical and forensic applications and a longer detection window afforded by monitoring these metabolites is obvious. LC-MS/MS is used to analyze diluted urine with deuterated analogs of each analyte as internal standards to ensure accurate quantitation and control for any potential matrix effects. High aqueous HPLC is used to chromatograph the metabolites. Negative ion electrospray is used to introduce the metabolites into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each metabolite, while a matrix-matched calibration curve is used for quantitation.

KEYWORDS:

Ethyl glucuronide; Ethyl sulfate; Mass spectrometry; Urine

PMID:
26660185
DOI:
10.1007/978-1-4939-3252-8_18
[Indexed for MEDLINE]

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