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Methods Mol Biol. 2016;1383:49-57. doi: 10.1007/978-1-4939-3252-8_6.

Quantitation of Haloperidol, Fluphenazine, Perphenazine, and Thiothixene in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Author information

1
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, 209-D02, Salt Lake City, UT, 84108, USA. matthew.slawson@aruplab.com.
2
ARUP Laboratories, Salt Lake City, UT, USA. matthew.slawson@aruplab.com.
3
Department of Pathology, University of Utah School ofMedicine, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, 209-D02, Salt Lake City, UT, 84108, USA.

Abstract

Haloperidol, fluphenazine, perphenazine, and thiothixene are "typical" antipsychotic drugs that are used in the treatment of schizophrenia and other psychiatric disorders. The monitoring of the use of these drugs has applications in therapeutic drug monitoring and overdose situations. LC-MS/MS is used to analyze plasma/serum extracts with deuterated analog of imipramine as the internal standard to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix-matched calibration curve is used for quantitation.

KEYWORDS:

Fluphenazine; Haloperidol; Mass spectrometry; Perphenazine; Plasma; Serum; Thiothixene; UPLC

PMID:
26660173
DOI:
10.1007/978-1-4939-3252-8_6
[Indexed for MEDLINE]

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