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Methods Mol Biol. 2016;1370:137-44. doi: 10.1007/978-1-4939-3142-2_11.

In Vivo Imaging of Microtubule Organization in Dividing Giant Cell.

Author information

1
Laboratoire de Reproduction et Développement des Plantes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
2
INRA, Institut Sophia Agrobiotech, UMR 1355, 400 route des Chappes, 06903, Sophia-Antipolis, France. favery@sophia.inra.fr.
3
CNRS, UMR 7254, 400 route des Chappes, 06903, Sophia-Antipolis, France. favery@sophia.inra.fr.
4
Université de Nice Sophia-Antipolis, UMR 1355, 400 route des Chappes, 06903, Sophia-Antipolis, France. favery@sophia.inra.fr.

Abstract

Mitosis which is a major step during plant development can also be observed in physiopathological conditions. During the compatible interaction between the root-knot nematode Meloidogyne incognita and its host Arabidopsis, the pathogen induce through repeated divisions without complete cytokinesis the formation of hypertrophied and multinucleate feeding cells, named giant cells. Due to the presence of hypertrophied plant cell material surrounding the giant cells, classical live cell imaging gave therefore very poor resolution. Here, we describe a protocol which allows the in vivo observation of the mitotic apparatus in developing giant cells using confocal imaging of vibrosliced tissues. This approach can also be used to visualize in vivo other cellular processes occurring in different steps of giant cells.

KEYWORDS:

Arabidopsis; Giant cells; Microtubule; Mitosis; Nematode

PMID:
26659960
DOI:
10.1007/978-1-4939-3142-2_11
[Indexed for MEDLINE]

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