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Methods Mol Biol. 2016;1370:51-7. doi: 10.1007/978-1-4939-3142-2_4.

Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis.

Aki SS1,2, Umeda M3,4.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
2
JST, CREST, Ikoma, Nara, 630-0192, Japan.
3
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan. mumeda@bs.naist.jp.
4
JST, CREST, Ikoma, Nara, 630-0192, Japan. mumeda@bs.naist.jp.

Abstract

This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system "Cytrap." The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green fluorescent protein (GFP) gene. We introduce growth conditions of Arabidopsis roots, the setup for microscopic observation, and analysis of obtained images.

KEYWORDS:

Arabidopsis; Cell cycle; Fluorescent protein marker; Real-time imaging; Root

PMID:
26659953
DOI:
10.1007/978-1-4939-3142-2_4
[Indexed for MEDLINE]

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