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Methods Mol Biol. 2016;1370:17-25. doi: 10.1007/978-1-4939-3142-2_2.

Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.

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Botany Department, School of Biology and Chemistry, Osnabrück University, Postfach 44 69, Osnabrück, 49069, Germany.


The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.


Agrobacterium; Cell division; Cytokinesis; F-actin; Green fluorescent protein; Tobacco BY-2; Transient transformation

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