Format

Send to

Choose Destination
Biol Reprod. 2016 Feb;94(2):30. doi: 10.1095/biolreprod.115.134692. Epub 2015 Dec 9.

The Effect of D-Aspartate on Spermatogenesis in Mouse Testis.

Author information

1
Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
2
Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Otsu, Shiga, Japan tanakah@belle.shiga-med.ac.jp.
3
Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Otsu, Shiga, Japan.
4
Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan kawauchi@belle.shiga-med.ac.jp.

Abstract

Spermatogenesis is controlled by hormonal secretions from the hypothalamus and pituitary gland, by factors produced locally in the testis, and by direct interaction between germ cells and Sertoli cells in seminiferous tubules. Although the mammalian testis contains high levels of D-aspartate (D-Asp), and D-Asp is known to stimulate the secretion of testosterone in cultured Leydig cells, its role in testis is unclear. We describe here biochemical, immunohistochemical, and flow cytometric studies designed to elucidate developmental changes in testicular D-Asp levels and the direct effect of D-Asp on germ cells. We found that the concentration of D-Asp in mouse testis increased with growth and that fluctuations in D-Asp levels were controlled in part by its degradative enzyme, D-aspartate oxidase expressed in Sertoli cells. In vitro sperm production studies showed that mitosis in premeiotic germ cells was strongly inhibited by the addition of D-Asp to the culture medium. Moreover, immunohistochemical analysis demonstrated that d-Asp accumulated in the differentiated spermatids, indicating either transport of D-Asp to spermatids or its de novo synthesis in these cells. Such compartmentation seems to prevent premeiotic germ cells in mouse testis from being exposed to the excess amount of D-Asp. In concert, our results indicate that in mouse testis, levels of D-Asp are regulated in a spatiotemporal manner and that D-Asp functions as a modulator of spermatogenesis.

KEYWORDS:

D-aspartate; Sertoli cell; spermatogenesis; testis

PMID:
26658710
DOI:
10.1095/biolreprod.115.134692
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center