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PLoS One. 2015 Dec 11;10(12):e0144597. doi: 10.1371/journal.pone.0144597. eCollection 2015.

Pax6 Inactivation in the Adult Pancreas Reveals Ghrelin as Endocrine Cell Maturation Marker.

Author information

1
Max Planck Institute for Biophysical Chemistry, Department of Molecular Developmental Biology, RG Molecular Cell Differentiation, Goettingen, Germany.
2
Université de Nice Sophia Antipolis, Nice, France.
3
Inserm U1091, IBV, Diabetes Genetics Team, Nice, France.
4
JDRF, New York, NY, United States of America.
5
Genome and Stem Cell Center, GENKOK, Erciyes University, Kayseri, Turkey.
6
University of Goettingen, Department of Clinical Neurophysiology, Goettingen, Germany.

Abstract

The transcription factor Pax6 is an important regulator of development and cell differentiation in various organs. Thus, Pax6 was shown to promote neural development in the cerebral cortex and spinal cord, and to control pancreatic endocrine cell genesis. However, the role of Pax6 in distinct endocrine cells of the adult pancreas has not been addressed. We report the conditional inactivation of Pax6 in insulin and glucagon producing cells of the adult mouse pancreas. In the absence of Pax6, beta- and alpha-cells lose their molecular maturation characteristics. Our findings provide strong evidence that Pax6 is responsible for the maturation of beta-, and alpha-cells, but not of delta-, and PP-cells. Moreover, lineage-tracing experiments demonstrate that Pax6-deficient beta- and alpha-cells are shunted towards ghrelin marked cells, sustaining the idea that ghrelin may represent a marker for endocrine cell maturation.

PMID:
26658466
PMCID:
PMC4676685
DOI:
10.1371/journal.pone.0144597
[Indexed for MEDLINE]
Free PMC Article

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