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Nat Methods. 2016 Feb;13(2):139-42. doi: 10.1038/nmeth.3690. Epub 2015 Dec 14.

Inverted light-sheet microscope for imaging mouse pre-implantation development.

Author information

1
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
2
Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.

PMID:
26657559
DOI:
10.1038/nmeth.3690
[Indexed for MEDLINE]

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