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Nat Methods. 2016 Feb;13(2):143-6. doi: 10.1038/nmeth.3689. Epub 2015 Dec 14.

Nanoscale optomechanical actuators for controlling mechanotransduction in living cells.

Author information

1
Department of Chemistry, Emory University, Atlanta, Georgia, USA.
2
Georgia Institute of Technology, George W. Woodruff School of Mechanical Engineering, Atlanta, Georgia, USA.
3
Georgia Institute of Technology, School of Materials Science and Engineering, Atlanta, Georgia, USA.
4
Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, USA.
5
AFM Business Unit, Bruker Nano Surfaces, Santa Barbara, California, USA.

Abstract

To control receptor tension optically at the cell surface, we developed an approach involving optomechanical actuator nanoparticles that are controlled with near-infrared light. Illumination leads to particle collapse, delivering piconewton forces to specific cell surface receptors with high spatial and temporal resolution. We demonstrate optomechanical actuation by controlling integrin-based focal adhesion formation, cell protrusion and migration, and T cell receptor activation.

PMID:
26657558
PMCID:
PMC4732909
DOI:
10.1038/nmeth.3689
[Indexed for MEDLINE]
Free PMC Article

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