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Nat Methods. 2016 Feb;13(2):147-50. doi: 10.1038/nmeth.3691. Epub 2015 Dec 14.

TRP channel mediated neuronal activation and ablation in freely behaving zebrafish.

Author information

1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
2
Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA.

Abstract

The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.

PMID:
26657556
PMCID:
PMC4851460
DOI:
10.1038/nmeth.3691
[Indexed for MEDLINE]
Free PMC Article
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