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Bioinformatics. 2016 Apr 15;32(8):1278-80. doi: 10.1093/bioinformatics/btv728. Epub 2015 Dec 10.

SpotCaliper: fast wavelet-based spot detection with accurate size estimation.

Author information

1
Biomedical Imaging Group, École polytechnique fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.

Abstract

MOTIVATION:

SpotCaliper is a novel wavelet-based image-analysis software providing a fast automatic detection scheme for circular patterns (spots), combined with the precise estimation of their size. It is implemented as an ImageJ plugin with a friendly user interface. The user is allowed to edit the results by modifying the measurements (in a semi-automated way), extract data for further analysis. The fine tuning of the detections includes the possibility of adjusting or removing the original detections, as well as adding further spots.

RESULTS:

The main advantage of the software is its ability to capture the size of spots in a fast and accurate way.

AVAILABILITY AND IMPLEMENTATION:

http://bigwww.epfl.ch/algorithms/spotcaliper/

CONTACT:

zsuzsanna.puspoki@epfl.ch

SUPPLEMENTARY INFORMATION:

Supplementary data are available at Bioinformatics online.

PMID:
26656569
DOI:
10.1093/bioinformatics/btv728
[Indexed for MEDLINE]

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