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G3 (Bethesda). 2015 Dec 12;6(2):377-90. doi: 10.1534/g3.115.023044.

Genetic Mapping of Resistance to Meloidogyne arenaria in Arachis stenosperma: A New Source of Nematode Resistance for Peanut.

Author information

1
Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810 soraya.bertioli@embrapa.br.
2
Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil.
3
Department of Nematology, University of California, Riverside, California 92521.
4
Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810.
5
Embrapa Rice and Beans, Rodovia GO-462, km 12 Zona Rural C.P. 179, Santo Antônio de Goiás, GO, 75375-000, Brazil.
6
Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810 University of Brasília, Institute of Biological Sciences, Campus Darcy Ribeiro, Brasília, DF, 70910-900, Brazil.

Abstract

Root-knot nematodes (RKN; Meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. The use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. Peanut (Arachis hypogaea) is infected by four species of RKN, the most damaging being M. arenaria, and commercial cultivars rely on a single source of resistance. In this study, we genetically characterize RKN resistance of the wild Arachis species A. stenosperma using a population of 93 recombinant inbred lines developed from a cross between A. duranensis and A. stenosperma. Four quantitative trait loci (QTL) located on linkage groups 02, 04, and 09 strongly influenced nematode root galling and egg production. Drought-related, domestication and agronomically relevant traits were also evaluated, revealing several QTL. Using the newly available Arachis genome sequence, easy-to-use KASP (kompetitive allele specific PCR) markers linked to the newly identified RKN resistance loci were developed and validated in a tetraploid context. Therefore, we consider that A. stenosperma has high potential as a new source of RKN resistance in peanut breeding programs.

KEYWORDS:

Arachis; QTL; drought; introgression; marker-assisted selection; peanut; root-knot nematode resistance; yield

PMID:
26656152
PMCID:
PMC4751557
DOI:
10.1534/g3.115.023044
[Indexed for MEDLINE]
Free PMC Article

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