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Nat Chem Biol. 2016 Feb;12(2):117-23. doi: 10.1038/nchembio.1981. Epub 2015 Dec 14.

Cyclophilin A promotes cell migration via the Abl-Crk signaling pathway.

Author information

1
Department of Biochemistry, Molecular Biology &Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
2
Center for Integrative Proteomics Research, Rutgers University, Piscataway, New Jersey, USA.
3
Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey, USA.
4
Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
5
Department of Biological Sciences, Rutgers University, Newark, New Jersey, USA.

Abstract

Cyclophilin A (CypA) is overexpressed in a number of human cancer types, but the mechanisms by which the protein promotes oncogenic properties of cells are not understood. Here we demonstrate that CypA binds the CrkII adaptor protein and prevents it from switching to the inhibited state. CrkII influences cell motility and invasion by mediating signaling through its SH2 and SH3 domains. CrkII Tyr221 phosphorylation by the Abl or EGFR kinases induces an inhibited state of CrkII by means of an intramolecular SH2-pTyr221 interaction, causing signaling interruption. We show that the CrkII phosphorylation site constitutes a binding site for CypA. Recruitment of CypA sterically restricts the accessibility of Tyr221 to kinases, thereby suppressing CrkII phosphorylation and promoting the active state. Structural, biophysical and in vivo data show that CypA augments CrkII-mediated signaling. A strong stimulation of cell migration is observed in cancer cells wherein both CypA and CrkII are greatly upregulated.

PMID:
26656091
PMCID:
PMC4718742
DOI:
10.1038/nchembio.1981
[Indexed for MEDLINE]
Free PMC Article

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