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Neurosci Lett. 2016 Feb 12;614:70-6. doi: 10.1016/j.neulet.2015.11.052. Epub 2015 Dec 3.

Expression and colocalization of NMDA receptor and FosB/ΔFosB in sensitive brain regions in rats after chronic morphine exposure.

Author information

1
Department of Genetics, Xuzhou Medical College, Xuzhou City, PR China.
2
Graduate school, Xuzhou Medical College, Xuzhou City, PR China.
3
Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China.
4
Research Facility Center for Morphology of Xuzhou Medical College, Xuzhou City, PR China.
5
Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming City, PR China. Electronic address: fudanhumin@sina.com.
6
Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China. Electronic address: oto8558@163.com.
7
Department of Genetics, Xuzhou Medical College, Xuzhou City, PR China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China. Electronic address: nhcnhc@163.com.

Abstract

Research in the last decade demonstrated that the NMDA receptor (NMDAR) has an important role in opiate-induced neural and behavioral plasticity. In addition, increased levels of FosB-like proteins (FosB/ΔFosB) were found to be related to morphine withdrawal behaviors. However, the relationship between NMDAR and FosB/ΔFosB in sensitive brain regions during morphine withdrawal is largely unknown. In this study, we aimed to investigate NMDAR dynamics and FosB/ΔFosB levels in multiple brain regions and whether they are related in sensitive brain regions during morphine abstinence. Quantitative immunohistochemistry was adopted to test NMDAR and FosB/ΔfosB levels during morphine withdrawal in rats. Increased NMDAR and FosB/ΔFosB levels were found in the nucleus accumbens core (AcbC), nucleus accumbens shell (AcbSh), central amygdaloid nucleuscapsular part (CeC), ventral tegmental area (VTA) and cingulate cortex (Cg). Double-immunofluorescence labeling indicated that NMDAR colocalized with Fos/ΔFosB in these five regions. These results suggest that multiple phenotypic regions are mediated by NMDAR and Fos/ΔFosB during morphine withdrawal, such as the motivational (AcbC, AcbSh), limbic (CeC, VTA) and executive (Cg) system pathways, and may be the primary targets of NMDAR and Fos/ΔfosB that impact morphine withdrawal behaviors.

KEYWORDS:

FosB proteins; Limbic system; Morphine; NMDA receptor; Withdrawal

PMID:
26655477
DOI:
10.1016/j.neulet.2015.11.052
[Indexed for MEDLINE]

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