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Int Endod J. 2017 Jan;50(1):48-57. doi: 10.1111/iej.12591. Epub 2016 Jan 27.

In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.

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Bioingénierie Tissulaire, University Bordeaux, Bordeaux, France.
Bioingénierie Tissulaire, INSERM, Bordeaux, France.
Service d'Odontologie et de Santé Buccale, CHU de Bordeaux, Bordeaux, France.



To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.


A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t-tests and the Mann-Whitney U test were performed.


The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).


The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.


adult stem cells; cell differentiation; endodontics; hydrogel; regenerative medicine; tissue engineering

[Indexed for MEDLINE]

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