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Int Endod J. 2017 Jan;50(1):48-57. doi: 10.1111/iej.12591. Epub 2016 Jan 27.

In vitro assessment of a collagen/alginate composite scaffold for regenerative endodontics.

Author information

1
Bioingénierie Tissulaire, University Bordeaux, Bordeaux, France.
2
Bioingénierie Tissulaire, INSERM, Bordeaux, France.
3
Service d'Odontologie et de Santé Buccale, CHU de Bordeaux, Bordeaux, France.

Abstract

AIM:

To develop a biological scaffold that could be moulded to reproduce the geometry of a gutta-percha point with precision and allow the differentiation of mesenchymal stem cells into osteoblasts to be used as a regenerative endodontic material.

METHODOLOGY:

A collagen/alginate composite scaffold was cast into a sodium alginate mould to produce a gutta-percha point-like cone. Prior to gelation, the cone was seeded with human stem cells from the apical papilla (SCAPs) to evaluate cell/scaffold interactions. The reconstructed tissue was characterized after 8 days in culture. Elastic modulus, tissue compaction and cell differentiation were assessed. Student t-tests and the Mann-Whitney U test were performed.

RESULTS:

The fabrication method developed enabled the shape of a gutta-percha point to be mimicked with great accuracy and reproducibility (P = 0.31). Stem cells seeded into this composite scaffold were able to spread, survive and proliferate (P < 0.001). Moreover, they were able to differentiate into osteoblasts and produce calcified osseous extracellular matrix (P < 0.001). The construct showed no significant contraction after 8 days, preserving its shape and tip diameter (P = 0.58).

CONCLUSIONS:

The composite scaffold could present substantial benefits compared to synthetic materials. It could provide a favourable healing environment in the root canal conducive for regenerative endodontics and is therefore appropriate to be evaluated in vivo in further studies.

KEYWORDS:

adult stem cells; cell differentiation; endodontics; hydrogel; regenerative medicine; tissue engineering

PMID:
26650723
DOI:
10.1111/iej.12591
[Indexed for MEDLINE]

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