Format

Send to

Choose Destination
Bioanalysis. 2016;8(1):43-53. doi: 10.4155/bio.15.228. Epub 2015 Dec 9.

Absolute quantification of 35 plasma biomarkers in human saliva using targeted MS.

Author information

1
Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
2
Centre Mémoire Ressources Recherche Languedoc-Roussillon, CHRU de Montpellier, hôpital Gui de Chauliac, Montpellier, France.
3
Université de Montpellier, Montpellier, France.

Abstract

BACKGROUND:

Although the use of human saliva for diagnosing disease has been known to be of great clinical potential, few attempts have been made so far to develop its use. In this work, we developed an MRM-MS approach for 35 plasma biomarkers using human saliva in a clinical environment.

METHODS & RESULTS:

A 30-min micro LC-MS/MS run in MRM mode was conducted in order to quantify the 35 plasma proteins in human saliva. Sample preparation procedures were performed in quadruplicate and analyzed in duplicate. Results show that 32 of the 35 plasma proteins were quantified in human saliva using calibration curves in the 2- log10 dynamic ranges with excellent linearity.

DISCUSSION/CONCLUSION:

Our MRM method is compatible with routine measurements in daily clinical practice.

KEYWORDS:

MS; biochemistry; clinical chemistry; proteomics; saliva

PMID:
26647646
DOI:
10.4155/bio.15.228
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center