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Pancreatology. 2016 Jan-Feb;16(1):38-43. doi: 10.1016/j.pan.2015.10.014. Epub 2015 Nov 18.

Long-term evolution of acinar-to-ductal metaplasia and β-cell mass after radiofrequency-assisted transection of the pancreas in a controlled large animal model.

Author information

1
Cancer Research Group HBP, Fundación Instituto Mar de Investigaciones Médicas, Doctor Aiguader 88, Barcelona 08003, Spain. Electronic address: rquesada@imim.es.
2
Medicine and Surgery of Animals Department, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona 08193, Spain.
3
General and Digestive Surgery Department, Hospital Universitari Sagrat Cor, Viladomat 288, 08029 Barcelona, Spain.
4
Department of Pathology, Hospital del Mar, Passeig Marítim 25-29, Barcelona 08003, Spain.
5
Hepatobiliary and Liver Transplant Surgical Unit, St. Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
6
General Surgery Department, Hospital del Mar, Passeig Marítim 25-29, Barcelona 08003, Spain.
7
Biomedical Synergy, Electronic Engineering Department, Universitat Politècnica de València, Valencia 46022, Spain.

Abstract

BACKGROUND:

Pancreatic duct ligation (PDL) has been used as a model of chronic pancreatitis and as a model to increase β-cell mass. However, studies in mice have demonstrated acinar regeneration after PDL, questioning the long-term validity of the model. We aim to elucidate whether RF-assisted transection (RFAT) of the main pancreatic duct is a reliable PDL model, both in short (ST, 1-month) and long-term (LT, 6-months) follow-ups.

METHODS:

Eleven pigs were subjected to RFAT. Biochemical (serum/peripancreatic amylase and glucose) and histological changes (including a semiautomatic morphometric study of over 1000 images/pancreas and IHC analysis) were evaluated after ST or LT follow-up and also in fresh pancreas specimens that were used as controls for 1 (n = 4) and 6 months (n = 6).

RESULTS:

The distal pancreas in the ST was characterized by areas of acinar-to-ductal metaplasia (56%) which were significantly reduced at LT (21%) by fibrotic replacement and adipose tissue. The endocrine mass showed a normal increase.

CONCLUSION:

RFAT in the pig seems to be an appropriate PDL model without restoration of pancreatic drainage or reduction of endocrine mass.

KEYWORDS:

Acinar-to-ductal metaplasia; Animal model; Duodenopancreatectomy; Pancreatic duct ligation; Radiofrequency-assisted transection; β-cell mass

PMID:
26639388
DOI:
10.1016/j.pan.2015.10.014
[Indexed for MEDLINE]

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