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Cell. 2015 Dec 3;163(6):1314-25. doi: 10.1016/j.cell.2015.11.007.

Microscopy-Based High-Content Screening.

Author information

1
Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany. Electronic address: m.boutros@dkfz.de.
2
Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

Abstract

Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, and complex organismal phenotypes. Recently, advances in imaging and image-analysis methodologies have accelerated large-scale perturbation screens. Here, we describe the state of the art for image-based screening experiments and delineate experimental approaches and image-analysis approaches as well as discussing challenges and future directions, including leveraging CRISPR/Cas9-mediated genome engineering.

PMID:
26638068
DOI:
10.1016/j.cell.2015.11.007
[Indexed for MEDLINE]
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