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ACS Nano. 2016 Jan 26;10(1):845-52. doi: 10.1021/acsnano.5b05944. Epub 2015 Dec 8.

Antimicrobial Susceptibility Test with Plasmonic Imaging and Tracking of Single Bacterial Motions on Nanometer Scale.

Syal K1, Iriya R1,2, Yang Y1,2, Yu H1, Wang S1,3, Haydel SE4,5, Chen HY3, Tao N1,3,2.

Author information

1
Center for Biosensors and Bioelectronics, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.
2
School of Electrical, Computer and Energy Engineering, Arizona State University , Tempe, Arizona 85287, United States.
3
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
4
Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.
5
School of Life Sciences, Arizona State University , Tempe, Arizona 85287, United States.

Abstract

Antimicrobial susceptibility tests (ASTs) are important for confirming susceptibility to empirical antibiotics and detecting resistance in bacterial isolates. Currently, most ASTs performed in clinical microbiology laboratories are based on bacterial culturing, which take days to complete for slowly growing microorganisms. A faster AST will reduce morbidity and mortality rates and help healthcare providers administer narrow spectrum antibiotics at the earliest possible treatment stage. We report the development of a nonculture-based AST using a plasmonic imaging and tracking (PIT) technology. We track the motion of individual bacterial cells tethered to a surface with nanometer (nm) precision and correlate the phenotypic motion with bacterial metabolism and antibiotic action. We show that antibiotic action significantly slows down bacterial motion, which can be quantified for development of a rapid phenotypic-based AST.

KEYWORDS:

bacterial metabolism; bacterial nanomotion; culture-free antimicrobial susceptibility tests; plasmonic imaging and tracking; real-time antimicrobial susceptibility tests; surface plasmon resonance microscopy

PMID:
26637243
DOI:
10.1021/acsnano.5b05944
[Indexed for MEDLINE]

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