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Pediatr Int. 2016 May;58(5):353-8. doi: 10.1111/ped.12855. Epub 2016 Feb 3.

Effect of insulin-like growth factor-I during the early postnatal period in intrauterine growth-restricted rats.

Author information

1
Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
2
Department of Pediatrics, Juntendo University Faculty of Medicine, Tokyo, Japan.
3
Neonatal Center, Juntendo University Shizuoka Hospital, Shizuoka, Japan.

Abstract

BACKGROUND:

Insulin-like growth factor-I (IGF-I) is essential for perinatal growth and development; low serum IGF-I has been observed during intrauterine growth restriction (IUGR). We investigated the effects of recombinant human (rh) IGF-I in IUGR rats during the early postnatal period.

METHODS:

Intrauterine growth restriction was induced by bilateral uterine artery ligation in pregnant rats. IUGR pups were divided into two groups injected daily with rhIGF-I (2 mg/kg; IUGR/IGF-I, n = 16) or saline (IUGR/physiologic saline solution (PSS), n = 16) from postnatal day (PND) 7 to 13. Maternal sham-operated pups injected with saline were used as controls (control, n = 16). Serum IGF-I and IGF binding proteins (IGFBP) 3 and 5 were measured on PND25. The expression of Igf-i, IGF-I receptor (Igf-ir), Igfbp3, and 5 mRNA in the liver and brain was measured using real-time polymerase chain reaction on PND25. Immunohistochemical staining of the liver for IGF expression was performed.

RESULTS:

Mean bodyweight on PND3 and PND25 in the IUGR pups (IUGR/IGF-I and IUGR/PSS) was significantly lower than that of the control pups. Serum IGF-I and hepatic Igf-ir mRNA in the IUGR pups were significantly lower than those in the control pups. In the IUGR/IGF-I group, hepatic Igfbp3 mRNA and liver immunohistochemical staining were increased. In the IUGR/PSS and control pups, there were no significant differences between these two groups in serum IGFBP3 and IGFBP5, hepatic Igf-i and Igfbp-5 mRNA, or brain Igf mRNA.

CONCLUSIONS:

No benefits on body and brain weight gain but an effective increase in hepatic IGFBP-3 was observed after treatment with 2 mg/kg rhIGF-I during the early postnatal period.

KEYWORDS:

catch-up growth; insulin-like growth factor; insulin-like growth factor binding protein; intrauterine growth restriction; recombinant human insulin-like growth factor-I

PMID:
26635331
DOI:
10.1111/ped.12855
[Indexed for MEDLINE]

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