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Nat Commun. 2015 Dec 4;6:8971. doi: 10.1038/ncomms9971.

Systematic pan-cancer analysis of tumour purity.

Author information

1
Institute for Computational Health Sciences, University of California, San Francisco, California 94158, USA.
2
Division of System Medicine, Department of Pediatrics, Stanford University, Stanford, California 94305, USA.

Abstract

The tumour microenvironment is the non-cancerous cells present in and around a tumour, including mainly immune cells, but also fibroblasts and cells that comprise supporting blood vessels. These non-cancerous components of the tumour may play an important role in cancer biology. They also have a strong influence on the genomic analysis of tumour samples, and may alter the biological interpretation of results. Here we present a systematic analysis using different measurement modalities of tumour purity in >10,000 samples across 21 cancer types from the Cancer Genome Atlas. Patients are stratified according to clinical features in an attempt to detect clinical differences driven by purity levels. We demonstrate the confounding effect of tumour purity on correlating and clustering tumours with transcriptomics data. Finally, using a differential expression method that accounts for tumour purity, we find an immunotherapy gene signature in several cancer types that is not detected by traditional differential expression analyses.

PMID:
26634437
PMCID:
PMC4671203
DOI:
10.1038/ncomms9971
[Indexed for MEDLINE]
Free PMC Article
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