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Int J Stem Cells. 2015 Nov;8(2):134-45. doi: 10.15283/ijsc.2015.8.2.134.

Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamster.

Author information

1
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
3
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
4
DVM graduated, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
5
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
6
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Department of Human Genetic, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
7
Hematology and Bone Marrow Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
8
Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

BACKGROUND:

Bone marrow-derived mesenchymal stem cells (BM-MSCs) have potential of differentiation and they secrete anti-inflammatory cytokines and growth factors which make them appropriate for cell therapy.

AIM OF THE WORK:

Were to evaluate the healing effect of BM-MSCs transplantation on germinal cells of busulfan-induced azoospermic hamsters.

MATERIAL AND METHODS:

In the present experimental case control study, BM-MSCs were isolated from bone marrow of donor albino hamsters. Five mature male recipient hamsters received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia, right testis of hamsters was injected with 10(6) BM-MSCs via efferent duct and the left one remained as azoospermia control testis. Five normal mature hamsters were selected as normal intact control. After 35 days, testes and epididymis of three groups were removed for histological evaluation.

RESULTS:

Histomorphological analyses of BM-MSCs treated testes and epididymis showed the epithelial tissue of seminiferous tubules had normal morphology and spermatozoa were present in epididymis tubes. Spermatogenesis was observed in most cell-treated seminiferous tubules. The untreated seminiferous tubules were empty.

CONCLUSION:

Transplanted BM-MSCs could successfully induce spermatogenesis in seminiferous tubules of azoospermic hamster. Therefore, BM-MSCs can be an attractive candidate in cell transplantation of azoospermia.

KEYWORDS:

Azoospermia; Bone marrow; Busulfan; Cell therapy; Hamster; Mesenchymal stem cell

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