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Mol Nutr Food Res. 2016 Mar;60(3):682-6. doi: 10.1002/mnfr.201500668. Epub 2015 Dec 29.

Coffee consumption rapidly reduces background DNA strand breaks in healthy humans: Results of a short-term repeated uptake intervention study.

Author information

1
Department of Chemistry, Division of Food Chemistry and Toxicology, Molecular Nutrition, University of Kaiserslautern, Kaiserslautern, Germany.
2
Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München, Freising, Bavaria, Germany.
3
Ds Statistik, Rosenthal-Bielatal, Germany.
4
Gruenstadt, Germany.

Abstract

SCOPE:

Intervention studies provide evidence that long-term coffee consumption correlates with reduced DNA background damage in healthy volunteers. Here, we report on short-term kinetics of this effect, showing a rapid onset after normal coffee intake.

METHODS AND RESULTS:

In a short-term human intervention study, we determined the effects of coffee intake on DNA integrity during 8 h. Healthy male subjects ingested coffee in 200 mL aliquots every second hour up to a total volume of 800 mL. Blood samples were taken at baseline, immediately before the first coffee intake and subsequently every 2 h, prior to the respective coffee intake. DNA integrity was assayed by the comet assay. The results show a significant (p < 0.05) reduction of background DNA strand breaks already 2 h after the first coffee intake. Continued coffee intake was associated with further decrements in background DNA damage within the 8 h intervention (p < 0.01 and p < 0.001, respectively). Mean tail intensities (TIs%) decreased from 0.33 TI% (baseline, 0 h) to 0.22 TI% (within 8 h coffee consumption).

CONCLUSION:

Repeated coffee consumption was associated with reduced background DNA strand breakage, clearly measurable as early as 2 h after first intake resulting in a cumulative overall reduction by about one-third of the baseline value.

KEYWORDS:

Coffee; Comet assay; DNA; Short-term; Strand breaks

PMID:
26632023
DOI:
10.1002/mnfr.201500668
[Indexed for MEDLINE]

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