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Int J Radiat Biol. 2016;92(1):1-10. doi: 10.3109/09553002.2016.1114188. Epub 2015 Dec 2.

Activity and expression of acetylcholinesterase in PC12 cells exposed to intermittent 1.8 GHz 217-GSM mobile phone signal.

Author information

1
a Interdepartmental Centre for Environmental Science Research, University of Bologna , Campus of Ravenna , Italy ;
2
b Department of Biological, Geological and Environmental Sciences , University of Bologna , Bologna , Italy ;
3
c Department of Physics , University of Bologna , Bologna , Italy.

Abstract

PURPOSE:

Due to its role in learning, memory and in many neurodegenerative diseases, acetylcholinesterase (AChE) represents an interesting endpoint to assess possible targets of exposure to radiofrequency electromagnetic fields (RF-EMF) generated by mobile phones. We investigated possible alterations of enzymatic activity, gene and protein expression of AChE in neuronal-like cells exposed to a 1.8 GHz Global System for Mobile Communication (GSM) modulated signal (217-GSM).

MATERIALS AND METHODS:

Rat PC12 cells were exposed for 24 h to 1.8 GHz 217-GSM signal. Specific adsorption rate (SAR) was 2 W/kg. AChE enzyme activity was assessed spectrophotometrically by Ellman's method, mRNA expression level was evaluated by real time polymerase chain reaction, and protein expression was assessed by Western blotting.

RESULTS:

AChE enzymatic activity increased of 1.4-fold in PC12 cells exposed to 217-GSM signal for 24 h, whilst AChE transcriptional or translational pathways were not affected.

CONCLUSION:

Our results provide the first evidence of effects on AChE activity after in vitro exposure of mammalian cells to the RF-EMF generated by GSM mobile phones, at the SAR value 2 W/kg. The obtained evidence promotes further investigations on AChE as a possible target of RF-EMF and confirm the ability of 1.8 GHz 217-GSM signal to induce biological effects in different mammalian cells.

KEYWORDS:

217-GSM signal; acetylcholinesterase activity; acetylcholinesterase gene and protein expression

PMID:
26630175
DOI:
10.3109/09553002.2016.1114188
[Indexed for MEDLINE]
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