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Spectrochim Acta A Mol Biomol Spectrosc. 2016 Mar 5;156:1-8. doi: 10.1016/j.saa.2015.11.014. Epub 2015 Nov 19.

Two-photon fluorescence and fluorescence imaging of two styryl heterocyclic dyes combined with DNA.

Author information

1
School of Materials Science and Engineering, Qilu University of Technology, Jinan 250353, China; Shandong Provincial Key Laboratory of Processing and Testing Technology of Glass and Functional Ceramics, Key Laboratory of Amorphous and Polycrystalline Materials, Shandong Provincial Key Laboratory of Fine Chemicals, Qilu University of Technology, Jinan 250353, China.
2
School of Materials Science and Engineering, Qilu University of Technology, Jinan 250353, China; Shandong Provincial Key Laboratory of Processing and Testing Technology of Glass and Functional Ceramics, Key Laboratory of Amorphous and Polycrystalline Materials, Shandong Provincial Key Laboratory of Fine Chemicals, Qilu University of Technology, Jinan 250353, China. Electronic address: zhangx@qlu.edu.cn.
3
Qilu Hospital of Shandong University, Jinan 250012, China.

Abstract

Two new styryl heterocyclic two-photon (TP) materials, 4-[4-(N-methyl)styrene]-imidazo [4,5-f][1,10] phenanthroline-benzene iodated salt (probe-1) and 4,4-[4-(N-methyl)styrene]-benzene iodated salt (probe-2) were successfully synthesized and studied as potential fluorescent probes of DNA detection. The linear and nonlinear photophysical properties of two compounds in different solvents were investigated. The absorption, one- and two-photon fluorescent spectra of the free dye and dye-DNA complex were also examined to evaluate their photophysical properties. The binding constants of dye-DNA were obtained according to Scatchard equation with good values. The results showed that two probes could be used as fluorescent DNA probes by two-photon excitation, and TP fluorescent properties of probe-1 are superior to that of probe-2. The fluorescent method date indicated that the mechanisms of dye-DNA complex interaction may be groove binding for probe-1 and electrostatic interaction for probe-2, respectively. The MTT assay experiments showed two probes are low toxicity. Moreover, the TP fluorescence imaging of DNA detection in living cells at 800 nm indicated that the ability to locate in cell nuclei of probe-1 is better than that of probe-2.

KEYWORDS:

DNA; Fluorescence imaging; Heterocyclic; Synthesize; Two-photon

PMID:
26629954
DOI:
10.1016/j.saa.2015.11.014
[Indexed for MEDLINE]

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