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EBioMedicine. 2015 Aug 15;2(10):1351-63. doi: 10.1016/j.ebiom.2015.08.026. eCollection 2015 Oct.

The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.

Author information

1
Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden.
2
Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N., PO Box 19024, Seattle, WA 98109, United States.
3
Department of Neuroscience, Uppsala University, Uppsala University Hospital, SE-751 85 Uppsala, Sweden.

Abstract

Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research.

KEYWORDS:

Cell culture; Glioblastoma; Molecular subtype; Stem cell culture condition; Xenograft models

PMID:
26629530
PMCID:
PMC4634360
DOI:
10.1016/j.ebiom.2015.08.026
[Indexed for MEDLINE]
Free PMC Article

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