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Int J Clin Exp Med. 2015 Sep 15;8(9):14896-905. eCollection 2015.

Berberine protects vascular endothelial cells in hypertensive rats.

Author information

  • 1Department of ECG, The First Affiliated Hospital, Harbin Medical University Harbin 150001, P. R. China.
  • 2Department of Orthopedic Surgery, The First Affiliated Hospital, Harbin Medical University Harbin 150001, P. R. China.

Abstract

OBJECTIVE:

This study is to investigate the effect and mechanism of berberine on vascular endothelial cell injury.

METHODS:

The isolated aortic endothelial cells were divided into negative control group, spontaneous hypertension group, and berberine group (1.25, 2.5, and 5 μmol/L berberine). CCK-8 assay was performed to detect cell proliferation. Annexin V-FITC flow cytometry and Hochest33342/PI staining were used to measure cell apoptosis. Expression of TLR4, Myd88, and NF-κB was detected with Western blotting analysis. Level of IL-6 and TNF-α was measured with ELISA.

RESULTS:

Compared with spontaneous hypertension group, cell proliferation in berberine group was significantly improved (P < 0.05). Flow cytometry showed that cell apoptosis was reduced in berberine group in a dose-dependent manner and there was statistically significant difference between spontaneous hypertension group and berberine group (P < 0.05). This result was further confirmed by Hochest33342/PI staining. Expression levels of TLR4, Myd88 and NF-κB were increased in spontaneous hypertension group. However, their expression levels were significantly reduced in berberine group than those in spontaneous hypertension group (P < 0.05). Similarly, levels of IL-6 and TNF-α were increased in spontaneous hypertension group and decreased in berberine group. And, the difference was significant (P < 0.05). Importantly, there were significant differences between negative control group and spontaneous hypertension group in cell proliferation, apoptosis, and expression of TLR4, Myd88, NF-κB, IL-6 and TNF-α.

CONCLUSION:

Berberine plays a protective role in vascular endothelial cell injury through inhibiting apoptosis and expression of TLR4, Myd88, NF-κB, IL-6 and TNF-α.

KEYWORDS:

Berberine; IL-6; Myd88; NF-κB; TLR4; TNF-α; spontaneous hypertension; vascular endothelial cell injury

PMID:
26628971
PMCID:
PMC4658860
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