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J Exp Bot. 2016 Feb;67(4):1123-35. doi: 10.1093/jxb/erv513. Epub 2015 Nov 30.

Transcriptomic and anatomical complexity of primary, seminal, and crown roots highlight root type-specific functional diversity in maize (Zea mays L.).

Author information

1
Institute of Crop Science and Resource Conservation, Crop Functional Genomics, University of Bonn, D-53113 Bonn, Germany.
2
Experimental Medicine and Therapy Research, University of Regensburg, D-93053 Regensburg, Germany.
3
Department of Statistics, Iowa State University, Ames, IA 50011-1210, USA.
4
Institute of Crop Science and Resource Conservation, Crop Functional Genomics, University of Bonn, D-53113 Bonn, Germany hochholdinger@uni-bonn.de.

Abstract

Maize develops a complex root system composed of embryonic and post-embryonic roots. Spatio-temporal differences in the formation of these root types imply specific functions during maize development. A comparative transcriptomic study of embryonic primary and seminal, and post-embryonic crown roots of the maize inbred line B73 by RNA sequencing along with anatomical studies were conducted early in development. Seminal roots displayed unique anatomical features, whereas the organization of primary and crown roots was similar. For instance, seminal roots displayed fewer cortical cell files and their stele contained more meta-xylem vessels. Global expression profiling revealed diverse patterns of gene activity across all root types and highlighted the unique transcriptome of seminal roots. While functions in cell remodeling and cell wall formation were prominent in primary and crown roots, stress-related genes and transcriptional regulators were over-represented in seminal roots, suggesting functional specialization of the different root types. Dynamic expression of lignin biosynthesis genes and histochemical staining suggested diversification of cell wall lignification among the three root types. Our findings highlight a cost-efficient anatomical structure and a unique expression profile of seminal roots of the maize inbred line B73 different from primary and crown roots.

KEYWORDS:

Crown root; RNA-seq; Zea mays.; primary root; root type; seminal root; transcriptome

PMID:
26628518
PMCID:
PMC4753849
DOI:
10.1093/jxb/erv513
[Indexed for MEDLINE]
Free PMC Article

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