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Cell Rep. 2015 Dec 15;13(10):2273-86. doi: 10.1016/j.celrep.2015.11.001. Epub 2015 Nov 25.

Intravacuolar Membranes Regulate CD8 T Cell Recognition of Membrane-Bound Toxoplasma gondii Protective Antigen.

Author information

1
INSERM, U1043, Toulouse 31300, France; CNRS, UMR 5282, Toulouse 31300, France; Centre de Physiopathologie de Toulouse Purpan (CPTP), Université de Toulouse, UPS, Toulouse 31300, France.
2
CNRS, UMR 5163, Grenoble 38000, France; Laboratoire Adaptation et Pathogénie des Microorganismes (LAPM), Université Grenoble Alpes, Grenoble 38000, France.
3
Institut de Biologie Structurale (IBS), Université Grenoble Alpes, Grenoble 38044, France; CNRS, IBS, Grenoble 38044, France; CEA, IBS, Grenoble 38044, France; CNRS, Unit for Virus Host-Cell Interactions (UVHCI), Grenoble 38042, France.
4
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
5
CNRS, UMR 5235, Université de Montpellier, Montpellier 34000, France.
6
INSERM, U1043, Toulouse 31300, France; CNRS, UMR 5282, Toulouse 31300, France; Centre de Physiopathologie de Toulouse Purpan (CPTP), Université de Toulouse, UPS, Toulouse 31300, France. Electronic address: nicolas.blanchard@inserm.fr.

Abstract

Apicomplexa parasites such as Toxoplasma gondii target effectors to and across the boundary of their parasitophorous vacuole (PV), resulting in host cell subversion and potential presentation by MHC class I molecules for CD8 T cell recognition. The host-parasite interface comprises the PV limiting membrane and a highly curved, membranous intravacuolar network (IVN) of uncertain function. Here, using a cell-free minimal system, we dissect how membrane tubules are shaped by the parasite effectors GRA2 and GRA6. We show that membrane association regulates access of the GRA6 protective antigen to the MHC I pathway in infected cells. Although insertion of GRA6 in the PV membrane is key for immunogenicity, association of GRA6 with the IVN limits presentation and curtails GRA6-specific CD8 responses in mice. Thus, membrane deformations of the PV regulate access of antigens to the MHC class I pathway, and the IVN may play a role in immune modulation.

PMID:
26628378
DOI:
10.1016/j.celrep.2015.11.001
[Indexed for MEDLINE]
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