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Am J Pathol. 2016 Jan;186(1):32-42. doi: 10.1016/j.ajpath.2015.09.019. Epub 2015 Nov 25.

Meibomian Gland Absence Related Dry Eye in Ectodysplasin A Mutant Mice.

Author information

1
Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
2
Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China; Department of Corneal & Ocular Surface Diseases, Xiamen University Affiliated Xiamen Eye Center, Xiamen, Fujian, China. Electronic address: zuguoliu@xmu.edu.cn.
3
Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China; Department of Corneal & Ocular Surface Diseases, Xiamen University Affiliated Xiamen Eye Center, Xiamen, Fujian, China. Electronic address: wei1018@xmu.edu.cn.

Abstract

Meibomian gland dysfunction is the most frequent cause of evaporative dry eye, yet its underlying pathophysiology is unknown. To gain insight into this pathophysiology, we characterized the time-dependent tear film and ocular surface changes occurring in X-linked anhidrotic-hypohidrotic ectodermal dysplasia mice (Tabby), which lack the meibomian gland. These mice sequentially developed corneal epithelial defects, central corneal stromal edema, neovascularization, and pannus 8 to 16 weeks after birth. Aqueous tear secretion was normal, whereas tear break-up time and ex vivo tear evaporation times were all shortened. Corneal epithelial microvilli were less numerous, conjunctival goblet cell density was unaffected, and MUC5AC and MUC5B gene expression was increased. Markers of squamous metaplasia (cytokeratin 10 and small proline-rich protein 1B) were noticed in the corneal epithelium of Tabby mice as early as the fourth week. Taken together, the Tabby mouse is a relevant meibomian gland dysfunction-related dry eye model that may lead to a better understanding of how meibomian glands are related to ocular surface health. This model may also help with discovering novel drug options for treating evaporative dry eye.

PMID:
26626448
DOI:
10.1016/j.ajpath.2015.09.019
[Indexed for MEDLINE]

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