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Bioanalysis. 2015 Dec;7(23):2991-3004. doi: 10.4155/bio.15.222. Epub 2015 Dec 2.

A standardized kit for automated quantitative assessment of candidate protein biomarkers in human plasma.

Author information

1
University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada.
2
Center for Proteomics & Metabolomics, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands.
3
Department of Biochemistry & Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada.

Abstract

BACKGROUND:

An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma.

DISCUSSION/CONCLUSION:

The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.

PMID:
26626236
DOI:
10.4155/bio.15.222
[Indexed for MEDLINE]
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