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Oncol Lett. 2015 Oct;10(4):2639-2643. Epub 2015 Jul 29.

microRNA-195 inhibits the proliferation, migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression.

Author information

1
Department of Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250100, P.R. China ; Department of Gynecology, Women and Children's Health Care Hospital of Linyi, Linyi, Shandong 276003, P.R. China.
2
Department of Gynecology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
3
Department of Gynecology, Women and Children's Health Care Hospital of Linyi, Linyi, Shandong 276003, P.R. China.
4
Department of Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250100, P.R. China.

Abstract

The functions of microRNAs (miRNA/miR) in the development of cervical cancer remain largely undefined. The present study investigated the role of miR-195 in cervical cancer development. The expression of miR-195 mimics in the cervical cancer HeLa cell line significantly decreased the cell proliferation, migration and invasion capacities in vitro. Using miRNA target prediction algorithms and reporter assays, cyclin D2 (CCND2) and v-myb avian myeloblastosis viral oncogene homolog (MYB) were identified as direct targets of miR-195. Moreover, miR-195 repressed the expression of CCND2 and MYB in the HeLa cells at the mRNA and protein levels. Finally, the expression of miR-195 was downregulated in cervical cancer tissues compared with normal tissues. Together, these data suggest that miR-195 is a tumor suppressor in cervical cancer.

KEYWORDS:

cervical cancer; invasion; microRNA-195; migration; proliferation

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