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Methods Mol Biol. 2015;1330:101-9. doi: 10.1007/978-1-4939-2848-4_10.

Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.

Author information

1
Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. fbninbar@gmail.com.
2
Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
3
San Diego Zoo Institute for Conservation Research, Escondido, CA, USA.
4
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.

Abstract

For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.

KEYWORDS:

Cellular reprogramming; Conservation; Endangered species; Induced pluripotent stem cells

PMID:
26621593
DOI:
10.1007/978-1-4939-2848-4_10
[Indexed for MEDLINE]

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