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Adv Exp Med Biol. 2015;883:1-20. doi: 10.1007/978-3-319-23603-2_1.

Metagenomics as a Tool for Enzyme Discovery: Hydrolytic Enzymes from Marine-Related Metagenomes.

Author information

1
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5G 1L6, Canada.
2
School of Biological Sciences, Bangor University, Gwynedd, LL57 2UW, UK.
3
Institute for Coastal Marine Environment, CNR, 98122, Messina, Italy.
4
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5G 1L6, Canada. a.iakounine@utoronto.ca.

Abstract

This chapter discusses metagenomics and its application for enzyme discovery, with a focus on hydrolytic enzymes from marine metagenomic libraries. With less than one percent of culturable microorganisms in the environment, metagenomics, or the collective study of community genetics, has opened up a rich pool of uncharacterized metabolic pathways, enzymes, and adaptations. This great untapped pool of genes provides the particularly exciting potential to mine for new biochemical activities or novel enzymes with activities tailored to peculiar sets of environmental conditions. Metagenomes also represent a huge reservoir of novel enzymes for applications in biocatalysis, biofuels, and bioremediation. Here we present the results of enzyme discovery for four enzyme activities, of particular industrial or environmental interest, including esterase/lipase, glycosyl hydrolase, protease and dehalogenase.

KEYWORDS:

Enzyme screening; Gene discovery; Gene library; Hydrolase; Metagenome

PMID:
26621459
DOI:
10.1007/978-3-319-23603-2_1
[Indexed for MEDLINE]

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