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Nat Commun. 2015 Dec 1;6:8782. doi: 10.1038/ncomms9782.

A redox signalling globin is essential for reproduction in Caenorhabditis elegans.

Author information

1
Department of Biology, Ghent University, Ghent B-9000, Belgium.
2
Department of Biomedical Sciences, University of Antwerp, Antwerp B-2000, Belgium.
3
Department of Chemistry, University of Antwerp, Antwerp B-2000, Belgium.
4
Department of Physics, University of Genova, Genova I-16146, Italy.
5
Department of Biosciences, University of Milano, Milano I-20133, Italy.
6
CNR-IBF and CIMAINA, University of Milano, Milano I-20133, Italy.

Abstract

Moderate levels of reactive oxygen species (ROS) are now recognized as redox signalling molecules. However, thus far, only mitochondria and NADPH oxidases have been identified as cellular sources of ROS in signalling. Here we identify a globin (GLB-12) that produces superoxide, a type of ROS, which serves as an essential signal for reproduction in C. elegans. We find that GLB-12 has an important role in the regulation of multiple aspects in germline development, including germ cell apoptosis. We further describe how GLB-12 displays specific molecular, biochemical and structural properties that allow this globin to act as a superoxide generator. In addition, both an intra- and extracellular superoxide dismutase act as key partners of GLB-12 to create a transmembrane redox signal. Our results show that a globin can function as a driving factor in redox signalling, and how this signal is regulated at the subcellular level by multiple control layers.

Comment in

PMID:
26621324
PMCID:
PMC4686822
DOI:
10.1038/ncomms9782
[Indexed for MEDLINE]
Free PMC Article

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