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Food Chem. 2016 Apr 15;197(Pt A):676-85. doi: 10.1016/j.foodchem.2015.10.104. Epub 2015 Oct 27.

Molecular interactions of a model bile salt and porcine bile with (1,3:1,4)-β-glucans and arabinoxylans probed by (13)C NMR and SAXS.

Author information

1
ARC Centre of Excellence in Plant Cell Walls and Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Hartley Teakle Building, St Lucia, Queensland 4072, Australia. Electronic address: nimagunness@uq.edu.au.
2
ARC Centre of Excellence in Plant Cell Walls and Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Hartley Teakle Building, St Lucia, Queensland 4072, Australia. Electronic address: b.flanagan@uq.edu.au.
3
Bragg Institute, Australian Nuclear Science and Technology Organisation (ANSTO), Locked Bag 2001, Kirrawee DC, NSW 2232, Australia. Electronic address: jitendra.mata@ansto.gov.au.
4
Bragg Institute, Australian Nuclear Science and Technology Organisation (ANSTO), Locked Bag 2001, Kirrawee DC, NSW 2232, Australia. Electronic address: epg@ansto.gov.au.
5
ARC Centre of Excellence in Plant Cell Walls and Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Hartley Teakle Building, St Lucia, Queensland 4072, Australia. Electronic address: m.gidley@uq.edu.au.

Abstract

Two main classes of interaction between soluble dietary fibres (SDFs), such as (1,3:1,4)-β-D-glucan (βG) and arabinoxylan (AX) and bile salt (BS) or diluted porcine bile, were identified by (13)C NMR and small angle X-ray scattering (SAXS). Small chemical shift differences of BS NMR resonances were consistent with effective local concentration or dilution of BS micelles mostly by βG, suggesting dynamic interactions; whilst the reduced line widths/intensities observed were mostly caused by wheat AX and the highest molecular size and concentrations of βG. SAXS showed evidence of changes in βG but not AX in the presence of BS micelles, at >13 nm length scale consistent with molecular level interactions. Thus intermolecular interactions between SDF and BS depend on both SDF source and its molecular weight and may occur alone or in combination.

KEYWORDS:

(1,3:1,4)-β-d-Glucan; (13)C NMR; Arabinoxylan; Bile salt; Glycochenodeoxycholic acid sodium salt (PubChem CID: 23696298); SAXS; Taurochenodeoxycholic acid sodium salt (PubChem CID: 23687512)

PMID:
26617003
DOI:
10.1016/j.foodchem.2015.10.104
[Indexed for MEDLINE]

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