Format

Send to

Choose Destination
Int J Biol Macromol. 2016 Feb;83:42-9. doi: 10.1016/j.ijbiomac.2015.11.049. Epub 2015 Nov 23.

Separation, characterization and anticancer activities of a sulfated polysaccharide from Undaria pinnatifida.

Author information

1
School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China. Electronic address: hykh888@163.com.
2
School of Chinese Medicine, Shandong College of Traditional Chinese Medicine, Yantai 264199, Shangdong, PR China.
3
Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
4
School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China.
5
Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China. Electronic address: xial_li@qq.com.

Abstract

The purpose of this paper was to investigate separation, characterization and anticancer activities of a sulfated polysaccharide (SPUP) from Undaria pinnatifida. Firstly, polysaccharide from U. pinnatifida was separated by DEAE-52 cellulose and Sephacryl S-400 column chromatography. As results, SPUP was obtained with the yield of 19.42%. Then, SPUP was characterized using chemical analysis, gas chromatography, size-exclusion HPLC chromatography, UV-vis spectra and FT-IR spectrum. The content of total sugar, uronic acid, protein and sulfate radical were 80.48%, 3.21%, 7.12% and 29.14%, respectively. SPUP was a heteropolysaccharide composed of fucose, glucose and galactose in a molar percentage of 27.15:19.34:53.51 with molecular weight of 97.9 kDa. Finally, the strongly against breast cancer activity of SPUP was confirmed by DMBA-induced breast cancer rats model. AS results, SPUP can significantly restrain breast abnormal enlargement, prolong tumor latency and reduced tumor incidence. Immunomodulatory activity and regulating abnormal sex hormones level might contribute to its anticancer activities.

KEYWORDS:

Characterization and anticancer activity; Polysaccharide; Separation; Undaria pinnatifida

PMID:
26616455
DOI:
10.1016/j.ijbiomac.2015.11.049
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center