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J Biomed Opt. 2016 Jul;21(7):71102. doi: 10.1117/1.JBO.21.7.071102.

Comparison study of distinguishing cancerous and normal prostate epithelial cells by confocal and polarization diffraction imaging.

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East Carolina University, Department of Physics, Greenville, North Carolina 27858, United States.
East Carolina University, Department of Internal Medicine, Brody School of Medicine, Greenville, North Carolina 27834, United States.
Tianjin University, Department of Biomedical Engineering, 92 Weijin Road, Tianjin 300072, China.
East Carolina University, Department of Computer Science, Greenville, North Carolina 27858, United States.


Accurate classification of malignant cells from benign ones can significantly enhance cancer diagnosis and prognosis by detection of circulating tumor cells (CTCs). We have investigated two approaches of quantitative morphology and polarization diffraction imaging on two prostate cell types to evaluate their feasibility as single-cell assay methods toward CTC detection after cell enrichment. The two cell types have been measured by a confocal imaging method to obtain their three-dimensional morphology parameters and by a polarization diffraction imaging flow cytometry (p-DIFC) method to obtain image texture parameters. The support vector machine algorithm was applied to examine the accuracy of cell classification with the morphology and diffraction image parameters. Despite larger mean values of cell and nuclear sizes of the cancerous prostate cells than the normal ones, it has been shown that the morphologic parameters cannot serve as effective classifiers. In contrast, accurate classification of the two prostate cell types can be achieved with high classification accuracies on measured data acquired separately in three measurements. These results provide strong evidence that the p-DIFC method has the potential to yield morphology-related “fingerprints” for accurate and label-free classification of the two prostate cell types.

[Indexed for MEDLINE]

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